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Only homozygous animals were being used in the analyses, regardless of whether heterozygous mice ended up run in behavioral experiments. Three cohorts of 11–sixteen mice underwent the primary battery of behavioral assays for a complete of forty two animals. All mice have been to begin with housed with their littermates of a similar intercourse during the Vanderbilt Murine Neurobehavioral Core, with managed twelve h mild/darkish cycles, freely out there food and water, and controlled temperature and humidity. The experimenter was at first blinded to the genotype of each animal, and every animal underwent exactly the same battery of behavioral assessments to determine phenotype.
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Our laboratory shown that SERT and αvβ3 receptors colocalize in midbrain presynaptic terminals (Mazalouskas et al., 2015), suggesting that SERT is localized to multiprotein complexes coordinated by integrin αvβ3 in midbrain synapses. Preceding reports suggest that in platelets, the Affiliation of SERT with activated αIIbβthree integrins, and its localization to adhesion complexes, are correlated with increased uptake exercise (Carneiro et al.
scores for synapses optimistic for SERT/talin colocalization (facts presented on the top of graph as the mean ± SEM; unpaired t
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